Cure for HIV/AIDS coming soon? Gene editing eliminates HIV infection in mice
The study by researchers at Temple University and the University of Pittsburgh boosts the confidence of scientists to eliminate the AIDS virus infection in humans.
New Delhi: US researchers have successfully removed HIV DNA from the genomes of living animals to remove HIV-1 infection using a gene editing tool, says a new study.
The study by researchers at Temple University and the University of Pittsburgh boosts the confidence of scientists to eliminate the HIV/AIDS virus infection in humans.
The team of researchers announced on Monday about performing the feat in three different animal models, including a "humanised" model in which mice were transplanted with human immune cells and infected with the virus.
The team is the first to demonstrate that HIV-1 replication can be completely shut down and the virus eliminated from infected cells in animals with a powerful gene editing technology known as CRISPR/Cas9.
The new work followed a previous proof-of-concept study it published in 2016, in which the researchers used transgenic rat and mouse models with HIV-1 DNA incorporated into the genome of every tissue of their bodies.
"Our new study is more comprehensive," Wenhui Hu, associate professor at the Temple University, who led the study, said in a statement.
"We confirmed the data from our previous work and improved the efficiency of our gene editing strategy. We also show that the strategy is effective in two additional mouse models, one representing acute infection in mouse cells and the other representing chronic, infection in human cells."
In the study, the team genetically inactivated HIV-1 in transgenic mice, reducing the RNA expression of viral genes by roughly 60 to 95 per cent, confirming their earlier findings.
They then tested their system in mice acutely infected with EcoHIV, the mouse equivalent of human HIV-1.
They found the excision efficiency of their strategy reached 96 per cent in EcoHIV mice, giving the first evidence for HIV-1 eradication by prophylactic treatment with the CRISPR/Cas9 system.
In the third animal model, latent HIV-1 infection was recapitulated in humanised mice engrafted with human immune cells, including T cells, followed by HIV-1 infection.
"These animals carry latent HIV in the genomes of human T cells, where the virus can escape detection," Hu explained.
The team now plans to repeat the study in primates, a more suitable animal model where HIV infection induces disease, with an eventual goal of conducting a clinical trial in human patients in the future.
The new findings have been published in the journal Molecular Therapy.
(With IANS inputs)