Immune system's NK cells linked to infant liver disease
Washington: Researchers at Cincinnati Children``s Hospital Medical Center have found that an overactive response by one of the immune system``s key weapons against infection - natural killer, or NK, cells - is linked to the onset of biliary atresia in infants, a disease where blocked bile ducts can cause severe liver damage and death.They also found that blocking a gene that helps NK cells attack bile duct tissues lessens damage and may be a way to treat the most common cause of chronically progressive liver disease in children.
"Our findings underscore the developing immune system`s role in causing injury to bile ducts soon after birth, and they have implications for developing new therapies to block the disease by targeting certain cells or pro-inflammatory circuits," said Jorge A. Bezerra, M.D., the study`s senior investigator and research director of the Division of Gastroenterology, Hepatology and Nutrition at Cincinnati Children`s.
Very little is known about the cause of biliary atresia, although it has been traced to the immune system responding to an infection in the liver and bile ducts.
Surface tissues inside the bile ducts are damaged, which in turn allows inflammatory cells to block the duct and the ongoing accumulation of fibrotic tissue.
To better understand the disease`s apparent link to the developing and still immature infant immune system, researchers in this study analyzed the livers of infants diagnosed with biliary atresia.
They found elevated populations of NK cells in the bile ducts. The NK cells over-expressed genes involved in creating substances that are cytotoxic, or toxic to living cells. This finding led the research team to experiment with a mouse model of biliary atresia.
In the mouse experiments, the researchers used a rotavirus infection to induce biliary atresia in newborn mice. Similar to what was observed in diseased human infant livers, the researchers found that active NK cells were the most abundant cells populating the mouse livers and bile ducts at the time of obstruction.
In addition, they found that NK cells rely on the receptor gene, Nkg2d, to make contact with and attack bile duct surface cells by attaching to the Nk2d protein, which resides on the membranes of bile duct cells.
Once that contact is established, NK cells break down the membranes of bile duct surface cells, leading to tissue damage.
When researchers blocked the Nkg2d receptor and depleted the number of NK cells, it prevented damage to bile duct surface tissues, even with the presence of rotavirus infection.
The continuity of the mouse pup bile ducts was maintained, bile was able to flow from the liver to the intestines, and the animals grew well into adulthood without liver-related symptoms.
The study, to be published in the Aug. 3 Journal of Clinical Investigation, is posted online on the journal``s website.