Raman spectroscopy modulated for broader biomedical applications
Washington: Scientists have now demonstrated the advantages of wavelength-modulated Raman spectroscopy, opening the door to wider biomedical and clinical applications such as real-time assessment of tissues during surgery.
The inelastic scattering of light from any sample is called the Raman Effect, named for the Nobel prize-winner C.V. Raman.
It yields a molecular fingerprint related to the intrinsic composition of the sample. With the advent of lasers for excitation, this analytical technique has been applied in many disciplines from mineral investigations to protein structure determination and single cell studies.
The technique enables cancerous lesions, which are accompanied by changes in chemical composition compared to normal tissue, to be detected as a vibrational spectroscopic fingerprint.
However, there are considerable challenges to using the method in a clinical setting because factors such as ambient light, background fluorescence, and `etaloning` (a phenomenon that degrades the performance of thinned, back-illuminated charge-coupled devices) can hinder the interpretation of images. Pre-processing the data is prone to introduce artefacts and seriously hamper a classification.
Scientists from St. Andrews (UK) and Jena (Germany) have now demonstrated that wavelength-modulated Raman spectroscopy, an alternative to standard Raman spectroscopy with monochromatic excitation, overcomes these key problems.
In this study they describe how to record Raman signals against a high auto-fluorescence background by studying liver tissue and record spectra of Paracetamol tablets in ambient light.
Corresponding author Christoph Krafft, PhD, of the Institute of Photonic Technology, Jena, Germany explained: "The principle of our implementation of wavelength-modulated Raman spectroscopy is that fluorescence emission, ambient light, and system transmission function do not significantly vary, whereas the Raman signals do vary upon multiple wavelength excitations with small wavelength shifts. In turn this leads us to `cleanly` extract the Raman signature even in the presence of such factors."
"In the current work, we developed a hardware-based approach to suppress confounding factors in Raman spectra that requires a minimum of pre-processing and offers further unsurpassed advantages," he added.
Their study has been published in Biomedical Spectroscopy and Imaging.
More from India
More from World
More from Sports
More from Entertaiment
- DNA : When will Indian govt take strong actions against terrorism?
- Drunk girl assaults Mumbai police in Worli
- Arun Jaitley upset with Subramaniam Swami's Twitter attack
- Bombay HC to pronounce verdict on entry of woman to Haji Ali Dargah today
- DNA: When will Indian govt take strong actions against terrorism? - Part II
- Brexit: Could Britain's withdrawal from EU still be averted?
- Islamic State bombings in southern Yemen kill 38
- UK pensions minister Crabb considering bid to succeed PM Cameron: Report
- Special panel on India's NSG membership bid? We're not aware, says China
- Ruby Rai, Bihar Class 12 topper now under arrest for cheating, says she never wanted to top the exam