Washington: Researchers at the University of California-Los Angeles (UCLA) have shed light on the role that the body’s immune system plays in bone loss.
The research team focused their study on low-density lipoprotein (LDL), the so-called "bad" cholesterol, and examined how high levels of oxidized LDL affect bone, and whether a type of immune cell called a T cell plays a role in the process. Using blood samples from healthy human volunteers, the team isolated the participants`` T cells and cultured them in a dish. Half of the T cells were combined with normal LDL, and the rest with oxidized LDL. The scientists stimulated half of the T cells to mimic an immune response, and left the other half alone. They found that the T cells exposed to oxidized LDL displayed a striking response. "Lo and behold, both the resting and the activated T cells started churning out a chemical that stimulates cells whose sole purpose is to destroy bone," Effros said. According to the researcher, the chemical called RANKL is involved in immune response and bone physiology. With a view to gaining a deeper understanding as to how the immune system participates in bone loss, the team repeated the experiment in a mouse model. Half the animals were fed a high-fat diet starting at one month of age, while the control group ate a normal diet. At 11 months, the mice on the high-fat diet showed elevated cholesterol and thinner bones. Upon testing the T cells of the mice on the high-fat diet, Effros and her colleagues found that the cells acted differently than those of the mice on the normal diet.The researchers said that the T cells switched on the gene that produces RANKL. They further revealed that the chemical also appeared in the animals`` bloodstream, suggesting that the cellular activity contributed to their bone loss. "It`s normal for our T cells to produce small amounts of RANKL during an immune response. But when RANKL is manufactured for long periods or at the wrong time, it results in excessive bone damage," Effros said. "That`s exactly what happened to the mice on the high-fat diet. The animals`` high cholesterol increased their levels of oxidized LDL, which told the T cells to keep generating RANKL. This discovery revealed to us how the immune system might play an entirely new role in bone loss," she added. Effros said that the next step would be exploring methods to control T cell response to oxidized LDL in an effort to develop immune-based approaches to prevent or slow bone loss. ANI