Pliskova, the 10th-seeded Czech playing in her first Grand Slam semi-final, triumphed 6-2, 7-6 (7/5).
Semis line-ups: Serena Williams vs Karolina Pliskova, Caroline Wozniacki vs Angelique Kerber; Novak Djokovic vs Gael Monfils, Stan Wawrinka vs Kei Nishikori
World number one Djokovic takes a 12-0 career lead over Monfils into their semi-final on Friday while Nishikori looks to build on his shock five-set win over Andy Murray against Wawrinka.
It was at the semi-final stage at Flushing Meadows last year that Williams crumbled under the pressure of chasing a rare calendar-year Grand Slam as she fell to eventual runner-up Roberta Vinci.
Stan Wawrinka will now lock horns with Kei Nishikori, who stunned Andy Murray to advance into semis.
Williams`s semi-final against Pliskova promises more fireworks. The 10th-seeded Czech leads the WTA tour in aces this year.
Murray looked to be in control at two sets to one ahead and carving out a break point in the third game of the fourth when a loud gong-like sound burst from the malfunctioning public address system in Arthur Ashe stadium.
The US Open first round match between Russia's Vitalia Diatchenko and Timea Bacsinszky of Switzerland is under investigation for suspicious betting patterns, it was confirmed today.
Pliskova will face either Serena Williams or Romanian Simona Halep for a place in Saturday`s championship match.
Murray, who followed up his third Grand Slam triumph at Wimbledon with Rio Olympics gold, takes on 2014 finalist Kei Nishikori for a place in the last four.
The RK Khanna tennis stadium in the capital could be packed to the rafters when Rafa is in town.
Wozniacki, the finalist in 2009 and 2014 and semi-finalist also in 2010 and 2011, sympathised with Sevastova after she spent almost three months out of the sport with a right ankle injury earlier this year.
Mirza`s serve came under severe threat, facing three break points, and was broken in the first instance itself. Garcia then held her serve easily to be on level terms at 5-5.
Novak Djokovic one will face another Frenchman, Gael Monfils, for a place in Sunday`s championship match after the 10th seed pummelled compatriot Lucas Pouille 6-4, 6-3, 6-3.
Louis Armstrong`s moving `What a Wonderful World` serenaded fans Tuesday as the US Open bid farewell to the stadium named after the legendary jazz trumpeter before it confronts the wrecking ball next week.
I listen to my coaches and my team-mates, the people around me, and I don`t really listen to what a lot of people out there are saying because a lot of it is rubbish.