Kolkata, Nov 05: With increasing cases of bacterial and fungal contamination being reported from foods and beverages across the country, new diagnostic kits developed for instant detection of many common pathogens in food products would now prove to be new weapons in the hands of consumers. Through multi-centric projects at premier national laboratories in Karnal, Lucknow, Mysore and New Delhi, kits for a number of common food-borne pathogens have been developed, the latest annual report of the Department of Biotechnology under the union Science and Technology Ministry said.
The National Dairy Research Institute, Karnal has successfully developed kits called Multiplex PCR and nested PCR assays combined with immuno-magnetic separation for rapid detection of two pathogens, E coli and Lysteria monocytogenes, commonly infecting milk and milk products.
"The assays have been successfully tested in market samples of milk and milk products and the results have been validated by routine microbiological analysis using selective media for respective pathogens," the report said.
Another PCR assay developed by Industrial Toxicology Research Centre, Lucknow, can not only track down the pathogens Shigella, Salmonella as well as E coli in a variety of food materials but also simultaneously differentiate them.
Similarly, the Central Food Technological Research Institute (CFTRI), Mysore has designed primers for genes targeting Bacillus cereus, Staphylococcus aureus and Yersinia enterocolitica responsible for various kinds of food poisoning.
Bureau Report