Proteins that inhibit HIV, Ebola release identified
Washington: In a surprise finding, scientists have discovered that a family of proteins that helps viruses, such as HIV and Ebola, enter a cell can also block the release of these deadly viruses.
"This is a surprising finding that provides new insights into our understanding of not only HIV infection, but also that of Ebola and other viruses," said Shan-Lu Liu, associate professor in the University of Missouri School of Medicine's Department of Molecular Microbiology and Immunology.
AIDS is a condition characterised by progressive failure of the immune system. It is caused by the human immunodeficiency virus type 1 (HIV-1).
When HIV-1 or any virus infects a cell, it replicates and spreads to other cells. One type of cellular protein - T cell immunoglobulin and mucin domain, or TIM-1 - has previously been shown to promote entry of some highly pathogenic viruses into host cells.
Now, researchers have found that the same protein possesses a unique ability to block the release of HIV-1 and Ebola virus.
"This study shows that TIM proteins keep viral particles from being released by the infected cell and instead keep them tethered to the cell surface," said Gordon Freeman, associate professor of medicine with Harvard Medical School's Dana-Farber Cancer Institute.
"This is true for several important enveloped viruses including HIV and Ebola. We may be able to use this insight to slow the production of these viruses," said Freeman.
Under the supervision of Liu, Minghua Li, a graduate student in the MU Pathobiology Area Programme, performed a series of experiments that revealed the protein's ability to inhibit HIV-1 release, resulting in diminished viral production and replication.
HIV-1 attacks cells that are vital to the body's immune system, such as T cells. These white blood cells play an important role in the body's response to infection, but HIV-1 disrupts the cells' ability to fight back against infection.
When the virus enters a host cell, it infects the cell and replicates, producing viral particles that spread to and infect other cells.
The researchers found that as the viral particles attempt to bud from, or leave, the infected cell, the TIM-family proteins located on the surface of the cell can attach to lipids on the surface of the viral particle.
These lipids - known as phosphatidylserine (PS) - are normally present on the inner side of the cellular membrane but can be exposed to the outer side upon viral infection.
When the TIM-family proteins come in contact with PS, the viral particle becomes attached to the host cell, keeping the particle from being released from the cell.
Because TIM-family proteins and PS are present on the surface of the cell and the viral particle, the viral particles get stuck to one another, forming a network of viral particles that accumulate on the surface of the host cell, rather than being released to infect other cells.
The study was published in the journal PNAS.